Ann Nesby was born in Joliet, Illinois. Both of her parents sang, with her father in a quartet and his mother in a gospel group. Both practiced at the house and Ann's mother helped teach her how to utilize her vocal abilities. As early as three, Ann was active in Macedonia Baptist Church where her father was a pastor.
In 1987, Ann cut a demo tape and visited her sister in Minneapolis. Her sister was a member of Sounds of Blackness and Ann was invited to sing with them. With Ann in the group, they were hired to Jimmy Jam and Terry Lewis's Perspective label.
In 1996, Nesby released her debut album I'm Here For You with production from Jimmy Jam and Terry Lewis. The album scored two dance hits with 'Can I Get A Witness' reaching #11 and 'Hold On' reaching #5 on the Dance charts. On the R&B charts, the single "I'll Do Anything For You" reached #51.
Nesby's second album, Put It On Paper was released in 2002 and had two big dance hits. 'Lovin Is Really My Game' topped the Dance charts and 'Love Is What We Need' reached #2. The album's title track also appeared on the R&B charts, reaching #44.
Make Me Better, her third album moved towards gospel and had similar success on the dance charts with two of its singles reaching the top 10 on the dance charts. 'Let Your Will Be Done (Remix)' reached #3 and 'Shelter' reached #6. During the same year, Nesby appeared in the film The Fighting Temptations.
2006 saw another gospel release by Ann, titled In The Spirit.
In 2007, Ann Nesby returned to R&B with her album This Is Love. The album's single "I Apologize" reached #61 on the Hot R&B Songs chart.