Jasmine Guy was born in 1964 in Boston, Massachusetts. Her father, Reverend William Guy, is African-American and her mother, Jaye Rudolph, is Portugese. She was raised in Atlanta Georgia where she attended Northside High School and went on to study dance at Alvin Ailey American Dance Center in New York City.
In 1990, Jasmine released her self-titled debut album. The album had three singles, all of which had reasonable success. "Try Me" was the first single released and reached #14 on the R&B charts. "Just Want To Hold You" followed and reached #34 on the Hot 100 and #27 on the R&B charts, and Jasmine Guy's final single, "Another Like My Lover" reached #9 on the R&B charts and #66 on the Hot 100.
During the same time as Jasmine released her album, she was part of the show A Different World. She starred in the show from 1987 to 1993 and developed a name for herself as the snooty, heavy-Southern-accented Whitley Gilbert. Through co-star Jada Pinkett Smith, Jasmine befriended rapper Tupac Shakur. After Tupac's death, Jasmine collaborated with Tupac's mother, Afeni, helping her to write a book about her life as a former Black Panther.
In film, Jasmine Guy has appeared in several films. She had a role in the movie Harlem Nights alongside Eddie Murphy and Richard Pryor. She also appeared on Spike Lee's School Daze. Her latest role was in the animated film Cats don't Dance.
In 2006, Jasmine mentioned that she would be recording a new album and would sell it online.